High Throughput Formation and Measurement of Occlusive Thrombosis in Porcine Blood
نویسندگان
چکیده
Understanding and treating cardiovascular disease can benefit greatly from the development of low volume, high throughput tools to study thrombosis. We have designed, fabricated, and validated a microfluidic device to induce shear rates from 4300-7100 s -1 using only 10 mL of whole blood for 4 simultaneous assays to measure occlusive thrombosis optically. Shear rates were confirmed by flow rate and particle imaging velocimetry. Thrombosis measurements showed similar acute growth rates for all shear rates while time to acute growth onset and occlusion depend on initial shear rate. Applications range from unravelling the blood clotting cascade to drug dosage response measurement.
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